First, let me say that defoliation is a ramification strategy. Your tree doesn't look to be developed to the point that you would want to start ramifying it. But, I understand, there is a need to know as in I want to do it to see what it does.
At the risk of being out in the weeds, let me guess that all live oaks are similar to cork oak in that new leaves emerge in spring and last year's leaves remain until fall. New leaves grow from the axils of the old leaves (i.e., where the petiole of last year's leaf joins the stem).
I don' think just removing last year's leaves will accomplish much. I think one ought wait until the new leaves have emerged and hardened. So, it is probably not too late - it is most likely too early. Either way, you can try defoliating a branch or two instead of the whole tree (gravest risk is that you kill a couple of branches instead of the whole tree) - branches are largely autonomous.
Alternatively, ask over at
IBC. Russel Coker is over there and is big on live oak bonsai.